func BenchmarkRoundtripFileBoom(b *testing.B) {
if *file == "" {
b.Skip("no file specified")
}
for i := 0; i < b.N; i++ {
br, err := boom.OpenBAM(*file)
if err != nil {
b.Fatalf("Open failed: %v", err)
}
f, err := os.OpenFile("/dev/null", os.O_APPEND|os.O_RDWR, 0666)
if err != nil {
b.Fatalf("Open failed: %v", err)
}
bw, err := boom.OpenBAMFile(f, "bw", br.Header())
if err != nil {
b.Fatalf("NewWriter failed: %v", err)
}
for {
r, _, err := br.Read()
if err != nil {
break
}
_, err = bw.Write(r)
if err != nil {
b.Fatalf("Write failed: %v", err)
}
}
br.Close()
f.Close()
}
}
func filterBam(in, out string, minId, minQ int, mapQb byte, minAvQ float64) error {
bf, err := boom.OpenBAM(in)
if err != nil {
return err
}
defer bf.Close()
bo, err := boom.CreateBAM(out, bf.Header(), true)
if err != nil {
return err
}
defer bo.Close()
for {
r, _, err := bf.Read()
if err != nil {
if err == io.EOF {
return nil
}
return err
}
if r.Score() >= mapQb && qualOk(r, minId, minQ, minAvQ) {
_, err = bo.Write(r)
if err != nil {
return err
}
}
}
}
func main() {
bf, err := boom.OpenBAM(in)
if err != nil {
fmt.Fprintln(os.Stderr, err)
os.Exit(1)
}
defer bf.Close()
names := bf.RefNames()
var classFilt []interval.IntTree
if annot != "" {
classFilt, err = filterFeats(annot, classes, names, thresh)
if err != nil {
fmt.Fprintln(os.Stderr, err)
os.Exit(1)
}
}
var reads, totals int
for {
r, _, err := bf.Read()
if err != nil {
if err == io.EOF {
break
}
fmt.Fprintln(os.Stderr, err)
os.Exit(1)
}
if r.Flags()&boom.Unmapped == 0 {
if qualOk(r, minId, minQ, minAvQ) {
totals++
if classFilt != nil && len(classFilt[r.RefID()].Get(intBam{r})) == 0 {
continue
}
if s := r.Score(); s >= mapQb && s != 0xff {
reads++
}
}
}
}
fmt.Println(in, reads, totals, float64(reads)/float64(totals))
}
func BenchmarkReadFileBoom(b *testing.B) {
if *file == "" {
b.Skip("no file specified")
}
for i := 0; i < b.N; i++ {
br, err := boom.OpenBAM(*file)
if err != nil {
b.Fatalf("Open failed: %v", err)
}
for {
_, _, err := br.Read()
if err != nil {
break
}
}
br.Close()
}
}
func checkNames(files set) ([]string, error) {
var names []string
for _, in := range files {
bf, err := boom.OpenBAM(in)
if err != nil {
return nil, err
}
if names != nil {
for i, n := range bf.RefNames() {
if names[i] != n {
return nil, errors.New("header mismatch")
}
}
}
names = bf.RefNames()
bf.Close()
}
return names, nil
}
func main() {
names, err := checkNames(reads)
if err != nil {
fmt.Fprintln(os.Stderr, err)
os.Exit(1)
}
feats, err := annotFeats(annot, classes, names)
if err != nil {
fmt.Fprintln(os.Stderr, err)
os.Exit(1)
}
var rc int
for _, in := range reads {
fmt.Fprintf(os.Stderr, "Reading %q\n", in)
bf, err := boom.OpenBAM(in)
if err != nil {
fmt.Fprintln(os.Stderr, err)
os.Exit(1)
}
for id := uintptr(0); ; id++ {
r, _, err := bf.Read()
if err != nil {
if err == io.EOF {
break
}
fmt.Fprintln(os.Stderr, err)
os.Exit(1)
}
rc++
if r.Flags()&boom.Unmapped == 0 {
feats[r.RefID()].DoMatching(func(iv interval.IntInterface) (done bool) {
f := iv.(intGff)
if f.FeatScore == nil {
f.FeatScore = new(float64)
}
*f.FeatScore += float64(r.Len())
return
}, intBam{r, id})
}
}
bf.Close()
}
var exp gffFeatures
for _, chr := range feats {
chr.Do(func(iv interval.IntInterface) (done bool) {
f := iv.(intGff)
if f.FeatScore == nil {
return
}
*f.FeatScore /= float64(rc) * float64(f.Len())
exp = append(exp, f.Feature)
return
})
}
sort.Sort(exp)
w := gff.NewWriter(os.Stdout, 60, false)
for _, f := range exp {
w.Write(f)
}
}
func main() {
names, err := checkNames(reads)
if err != nil {
fmt.Fprintln(os.Stderr, err)
os.Exit(1)
}
feats, err := annotFeats(annot, classes, names)
if err != nil {
fmt.Fprintln(os.Stderr, err)
os.Exit(1)
}
vm := make(map[string]*vector)
fm := make(map[string]string)
seen := make(map[*gff.Feature]struct{})
for _, in := range reads {
fmt.Fprintf(os.Stderr, "Reading %q\n", in)
bf, err := boom.OpenBAM(in)
if err != nil {
fmt.Fprintln(os.Stderr, err)
os.Exit(1)
}
loop:
for {
r, _, err := bf.Read()
if err != nil {
if err == io.EOF {
break
}
fmt.Fprintln(os.Stderr, err)
os.Exit(1)
}
switch filter {
case all:
case primary:
if !isPrimary(r) {
continue loop
}
if strict && isSecondary(r) {
continue loop
}
case secondary:
if !isSecondary(r) {
continue loop
}
if strict && isPrimary(r) {
continue loop
}
default:
panic(fmt.Errorf("illegal filter %d", filter))
}
if r.Flags()&boom.Unmapped == 0 {
feats[r.RefID()].DoMatching(func(iv interval.IntInterface) (done bool) {
f := iv.(intGff)
if _, ok := seen[f.Feature]; ok {
return
}
seen[f.Feature] = struct{}{}
if f.Feature.Feature != "repeat" {
return
}
att := f.FeatAttributes.Get("repeat")
if att == "" {
return
}
fields := strings.Fields(att)
s := mustAtoi(fields[2])
e := mustAtoi(fields[3])
v, ok := vm[fields[0]]
if !ok {
v = &vector{}
vm[fields[0]] = v
fm[fields[0]] = fields[1]
}
for i := e; i >= s; i-- {
v.inc(i)
}
return
}, intBam{r})
}
}
bf.Close()
}
for typ, vec := range vm {
for pos, val := range *vec {
fmt.Printf("%s\t%s\t%d\t%d\n", fm[typ], typ, pos, val)
}
}
}
func main() {
names, err := checkNames(reads)
if err != nil {
fmt.Fprintln(os.Stderr, err)
os.Exit(1)
}
feats, err := annotFeats(annot, classes, names)
if err != nil {
fmt.Fprintln(os.Stderr, err)
os.Exit(1)
}
vm := make(map[string]*vector)
fm := make(map[string]string)
for _, in := range reads {
fmt.Fprintf(os.Stderr, "Reading %q\n", in)
bf, err := boom.OpenBAM(in)
if err != nil {
fmt.Fprintln(os.Stderr, err)
os.Exit(1)
}
loop:
for {
r, _, err := bf.Read()
if err != nil {
if err == io.EOF {
break
}
fmt.Fprintln(os.Stderr, err)
os.Exit(1)
}
switch filter {
case all:
case primary:
if !isPrimary(r) {
continue loop
}
if strict && isSecondary(r) {
continue loop
}
case secondary:
if !isSecondary(r) {
continue loop
}
if strict && isPrimary(r) {
continue loop
}
default:
panic(fmt.Errorf("illegal filter %d", filter))
}
if r.Flags()&boom.Unmapped == 0 {
feats[r.RefID()].DoMatching(func(iv interval.IntInterface) (done bool) {
f := iv.(intGff)
repeatFields := strings.Fields(f.FeatAttributes.Get("repeat"))
if len(repeatFields) == 0 {
return
}
from, err := strconv.Atoi(repeatFields[2])
if err != nil {
panic(err)
}
v, ok := vm[repeatFields[0]]
if !ok {
v = &vector{}
vm[repeatFields[0]] = v
fm[repeatFields[0]] = repeatFields[1]
}
for pos := r.Start(); pos < r.End(); pos++ {
var loc int
switch f.FeatStrand {
case seq.Plus:
loc = pos - f.FeatStart + from
case seq.Minus:
loc = f.FeatEnd - pos + from
default:
return
}
var st seq.Strand
if r.Flags()&boom.Reverse != 0 {
st = -f.FeatStrand
} else {
st = f.FeatStrand
}
v.inc(loc, st)
}
return
}, intBam{r})
}
}
bf.Close()
}
for typ, vec := range vm {
for pos, val := range *vec {
if val[0] != 0 {
fmt.Printf("%s\t%s\tminus\t%d\t%d\n", fm[typ], typ, pos, val[0])
}
if val[1] != 0 {
fmt.Printf("%s\t%s\tplus\t%d\t%d\n", fm[typ], typ, pos, val[1])
}
}
}
}
