func Steps(params Parameters, inputs Inputs) Outputs {
var outputs Outputs
ctx := execution.GetContext()
em := ctx.EquipmentManager
rqout := em.MakeDeviceRequest("liquidhandler", "Manual")
response := <-rqout
// handle response problems
if response["status"] == "FAIL" {
log.Fatal("Error requesting liquid handler service")
}
liquidhandler := response["devicequeue"].(*execution.LiquidHandlingService)
samples := make([]*wtype.LHComponent, 0)
buffersample := mixer.SampleForTotalVolume(inputs.Buffer, params.Reactionvolume)
samples = append(samples, buffersample)
atpsample := mixer.Sample(inputs.ATP, params.Atpvol)
samples = append(samples, atpsample)
vectorsample := mixer.SampleForConcentration(inputs.Vector, params.Vectorconc)
samples = append(samples, vectorsample)
for _, part := range inputs.Parts {
partsample := mixer.SampleForConcentration(part, params.Partconc)
samples = append(samples, partsample)
}
resample := mixer.Sample(inputs.RestrictionEnzyme, params.Revol)
samples = append(samples, resample)
ligsample := mixer.Sample(inputs.Ligase, params.Ligvol)
samples = append(samples, ligsample)
reaction := mixer.MixInto(inputs.Outplate, samples...)
// set the block ID
reaction.BlockID = string(params.BlockID)
rq2 := liquidhandler.MakeMixRequest(reaction)
if rq2 == nil {
log.Fatal("Error running liquid handling request")
}
rq2.Tip_Type = inputs.TipType
liquidhandler.Run()
// incubate the reaction mixtures
incubate(reaction, params.Reactiontemp, params.Reactiontime, false)
// inactivate
incubate(reaction, params.Inactivationtemp, params.Inactivationtime, false)
// all done
outputs.Reaction = reaction
return outputs
}
func (w *Wrapper) MixInto(outplate *wtype.LHPlate, components ...*wtype.LHComponent) *wtype.LHSolution {
if !w.usedMix {
ctx := GetContext()
em := ctx.EquipmentManager
/*
cfg := ctx.ConfigService.GetConfig(w.threadID)
devname := ""
_, ok := cfg["LIQUIDHANDLER"]
if ok {
devname = cfg["LIQUIDHANDLER"].(string)
}
rqOut := em.MakeDeviceRequest("liquidhandler", devname)
*/
rqOut := em.MakeDeviceRequest("liquidhandler", "Manual")
response := <-rqOut
if response["status"] == "FAIL" {
log.Fatal("Error requesting liquid handler service")
}
w.liquidHandler = response["devicequeue"].(*LiquidHandlingService)
w.usedMix = true
}
reaction := mixer.MixInto(outplate, components...)
reaction.BlockID = string(w.threadID)
req := w.liquidHandler.MakeMixRequest(reaction)
if req == nil {
log.Fatal("Error running liquid handling request")
}
req.Tip_Type = w.tipType
w.liquidHandler.Run()
return reaction
}
