func Steps(params Parameters, inputs Inputs) Outputs {
var outputs Outputs
ctx := execution.GetContext()
em := ctx.EquipmentManager
rqout := em.MakeDeviceRequest("liquidhandler", "Manual")
response := <-rqout
// handle response problems
if response["status"] == "FAIL" {
log.Fatal("Error requesting liquid handler service")
}
liquidhandler := response["devicequeue"].(*execution.LiquidHandlingService)
samples := make([]*wtype.LHComponent, 0)
buffersample := mixer.SampleForTotalVolume(inputs.Buffer, params.Reactionvolume)
samples = append(samples, buffersample)
atpsample := mixer.Sample(inputs.ATP, params.Atpvol)
samples = append(samples, atpsample)
vectorsample := mixer.SampleForConcentration(inputs.Vector, params.Vectorconc)
samples = append(samples, vectorsample)
for _, part := range inputs.Parts {
partsample := mixer.SampleForConcentration(part, params.Partconc)
samples = append(samples, partsample)
}
resample := mixer.Sample(inputs.RestrictionEnzyme, params.Revol)
samples = append(samples, resample)
ligsample := mixer.Sample(inputs.Ligase, params.Ligvol)
samples = append(samples, ligsample)
reaction := mixer.MixInto(inputs.Outplate, samples...)
// set the block ID
reaction.BlockID = string(params.BlockID)
rq2 := liquidhandler.MakeMixRequest(reaction)
if rq2 == nil {
log.Fatal("Error running liquid handling request")
}
rq2.Tip_Type = inputs.TipType
liquidhandler.Run()
// incubate the reaction mixtures
incubate(reaction, params.Reactiontemp, params.Reactiontime, false)
// inactivate
incubate(reaction, params.Inactivationtemp, params.Inactivationtime, false)
// all done
outputs.Reaction = reaction
return outputs
}
// The core process for this protocol, with the steps to be performed
// for every input
func (e *TypeIISConstructAssembly) steps(p TypeIISConstructAssemblyParamBlock, r *TypeIISConstructAssemblyResultBlock) {
_wrapper := execution.NewWrapper(p.ID)
_ = _wrapper
samples := make([]*wtype.LHComponent, 0)
bufferSample := mixer.SampleForTotalVolume(p.Buffer, p.ReactionVolume)
samples = append(samples, bufferSample)
atpSample := mixer.Sample(p.Atp, p.AtpVol)
samples = append(samples, atpSample)
vectorSample := mixer.SampleForConcentration(p.Vector, p.VectorConc)
samples = append(samples, vectorSample)
for _, part := range p.Parts {
partSample := mixer.SampleForConcentration(part, p.PartConc)
samples = append(samples, partSample)
}
reSample := mixer.Sample(p.RestrictionEnzyme, p.ReVol)
samples = append(samples, reSample)
ligSample := mixer.Sample(p.Ligase, p.LigVol)
samples = append(samples, ligSample)
reaction := _wrapper.MixInto(p.OutPlate, samples...)
// incubate the reaction mixture
_wrapper.Incubate(reaction, p.ReactionTemp, p.ReactionTime, false)
// inactivate
_wrapper.Incubate(reaction, p.InactivationTemp, p.InactivationTime, false)
// all done
r.Reaction = reaction
}