Esempio n. 1
0
func (lhs *LiquidHandlingService) MakeMixRequest(solution *wtype.LHSolution) *liquidhandling.LHRequest {
	lhs.lock.Lock()
	defer lhs.lock.Unlock()
	rq, ok := lhs.RequestQueue[execute.ThreadID(solution.BlockID)]

	if !ok {
		// if we don't have a request with this ID, make a new one
		rq = liquidhandling.NewLHRequest()
		rq = initRequest(rq, execute.ThreadID(solution.BlockID))
	}

	if solution.Platetype != "" {
		rq.Output_platetype = factory.GetPlateByType(solution.Platetype)
	}
	rq.Output_solutions[solution.ID] = solution
	lhs.RequestQueue[execute.ThreadID(rq.BlockID)] = rq

	return rq
}
Esempio n. 2
0
// Runs a workflow for one sample
func (w *Workflow) Run(cf *Config) ([]interface{}, error) {
	id, err := uuid.NewV4()
	if err != nil {
		return nil, err
	}

	tid := execute.ThreadID(id.String())
	ctx := execution.GetContext()
	ctx.ConfigService.SetConfig(tid, cf.Config)

	wr, err := NewWorkflowRun(*id, w, cf)
	if err != nil {
		return nil, err
	}

	var errors []error
	go func() {
		for v := range wr.Errors {
			if v != nil {
				errors = append(errors, v)
			}
		}
	}()

	var messages []interface{}
	go func() {
		for v := range wr.Messages {
			//			messages = append(messages, fmt.Sprintf("%v", v.Value))
			messages = append(messages, v.Value)
		}
	}()

	<-wr.Done

	if len(errors) > 0 {
		return messages, Errors(errors)
	}
	return messages, nil
}
Esempio n. 3
0
func main() {
	eid, _ := uuid.NewV4()
	em := equipmentManager.NewAnthaEquipmentManager(eid.String())
	defer em.Shutdown()
	eem := equipmentManager.EquipmentManager(em)
	equipmentManager.SetEquipmentManager(&eem)
	//manual driver equipment
	mid, _ := uuid.NewV4()
	var mde equipment.Equipment
	var amd manual.AnthaManual
	amd = *manual.NewAnthaManual(mid.String())
	mde = amd
	em.RegisterEquipment(&mde)

	//cui logger middleware
	cmw := middleware.NewLogToCui(&amd.Cui)
	log_id, _ := uuid.NewV4()
	l := logger.NewAnthaFileLogger(log_id.String())
	l.RegisterMiddleware(cmw)
	var params Parameters
	var inputs Inputs

	// give this thing an arbitrary ID for testing

	id := execute.ThreadID(fmt.Sprintf("EXPERIMENT_1_%s", string(eid.String()[1:5])))

	fmt.Println(id)

	// set up parameters and inputs

	params.Reactionvolume = wunit.NewVolume(20, "ul")
	params.Partconc = wunit.NewConcentration(0.0001, "g/l")
	params.Vectorconc = wunit.NewConcentration(0.001, "g/l")
	params.Atpvol = wunit.NewVolume(1, "ul")
	params.Revol = wunit.NewVolume(1, "ul")
	params.Ligvol = wunit.NewVolume(1, "ul")
	params.Reactiontemp = wunit.NewTemperature(25, "C")
	params.Reactiontime = wunit.NewTime(1800, "s")
	params.Inactivationtemp = wunit.NewTemperature(40, "C")
	params.Inactivationtime = wunit.NewTime(60, "s")
	params.BlockID = id

	inputs.Parts = make([]*wtype.LHComponent, 4)

	for i := 0; i < 4; i++ {
		inputs.Parts[i] = factory.GetComponentByType("dna_part")
		inputs.Parts[i].CName = inputs.Parts[i].CName + "_" + strconv.Itoa(i+1)
	}

	inputs.Vector = factory.GetComponentByType("standard_cloning_vector_mark_1")
	inputs.RestrictionEnzyme = factory.GetComponentByType("SapI")
	inputs.Ligase = factory.GetComponentByType("T4Ligase")
	inputs.Buffer = factory.GetComponentByType("CutsmartBuffer")
	inputs.ATP = factory.GetComponentByType("ATP")
	inputs.Outplate = factory.GetPlateByType("pcrplate")
	inputs.TipType = factory.GetTipboxByType("Gilson50")

	ctx := execution.GetContext()
	conf := make(map[string]interface{})
	conf["MAX_N_PLATES"] = 1.5
	conf["MAX_N_WELLS"] = 12.0
	conf["RESIDUAL_VOLUME_WEIGHT"] = 1.0
	conf["SQLITE_FILE_IN"] = "/Users/msadowski/synthace/protocol_language/checkout/synthace-antha/anthalib/driver/liquidhandling/pm_driver/default.sqlite"
	conf["SQLITE_FILE_OUT"] = "/tmp/output_file.sqlite"
	ctx.ConfigService.SetConfig(id, conf)
	outputs := Steps(params, inputs)
	fmt.Println(outputs.Reaction)
}
Esempio n. 4
0
func NewWorkflowRun(id uuid.UUID, wf *Workflow, cf *Config) (*WorkflowRun, error) {
	params := make(map[flow.FullPortName]interface{})
	for _, port := range wf.InPorts {
		param, ok := cf.Parameters[port.Proc]
		if !ok {
			return nil, fmt.Errorf("required parameter not found %v", port)
		}

		// Need to unpack fields to map entries; use reflection to avoid
		// copying pointer based structures
		pv := reflect.ValueOf(param)
		fv := pv.Elem().FieldByName(port.Port)
		if !fv.IsValid() {
			return nil, fmt.Errorf("required parameter not found %v", port)
		}
		reflect.ValueOf(params).SetMapIndex(reflect.ValueOf(port), fv)
	}

	ins := make(map[flow.FullPortName]chan execute.ThreadParam)
	outs := make(map[flow.FullPortName]chan execute.ThreadParam)
	for _, port := range wf.InPorts {
		ins[port] = make(chan execute.ThreadParam)
		wf.Graph.SetInPort(fmt.Sprintf("%s.%s", port.Proc, port.Port), ins[port])
	}
	for _, port := range wf.OutPorts {
		outs[port] = make(chan execute.ThreadParam)
		wf.Graph.SetOutPort(fmt.Sprintf("%s.%s", port.Proc, port.Port), outs[port])
	}

	messages := make(chan execute.ThreadParam)
	errors := make(chan error)
	done := make(chan bool)

	// Point of no return... start running workflow
	flow.RunNet(&wf.Graph)

	for _, ch := range outs {
		go func(ch chan execute.ThreadParam) {
			for v := range ch {
				messages <- v
			}
		}(ch)
	}

	tid := execute.ThreadID(id.String())
	go func() {
		defer func() {
			for _, ch := range ins {
				close(ch)
			}
		}()

		for port, v := range params {
			param := execute.ThreadParam{
				Value: v,
				ID:    tid,
			}
			ins[port] <- param
		}
	}()

	go func() {
		<-wf.Graph.Wait()
		close(errors)
		close(messages)
		done <- true
	}()

	return &WorkflowRun{
		ID:       id,
		Outs:     outs,
		Ins:      ins,
		Messages: messages,
		Errors:   errors,
		Done:     done,
	}, nil
}