// The core process for this protocol, with the steps to be performed
// for every input
func (e *TypeIISConstructAssembly) steps(p TypeIISConstructAssemblyParamBlock, r *TypeIISConstructAssemblyResultBlock) {
_wrapper := execution.NewWrapper(p.ID)
_ = _wrapper
samples := make([]*wtype.LHComponent, 0)
bufferSample := mixer.SampleForTotalVolume(p.Buffer, p.ReactionVolume)
samples = append(samples, bufferSample)
atpSample := mixer.Sample(p.Atp, p.AtpVol)
samples = append(samples, atpSample)
vectorSample := mixer.SampleForConcentration(p.Vector, p.VectorConc)
samples = append(samples, vectorSample)
for _, part := range p.Parts {
partSample := mixer.SampleForConcentration(part, p.PartConc)
samples = append(samples, partSample)
}
reSample := mixer.Sample(p.RestrictionEnzyme, p.ReVol)
samples = append(samples, reSample)
ligSample := mixer.Sample(p.Ligase, p.LigVol)
samples = append(samples, ligSample)
reaction := _wrapper.MixInto(p.OutPlate, samples...)
// incubate the reaction mixture
_wrapper.Incubate(reaction, p.ReactionTemp, p.ReactionTime, false)
// inactivate
_wrapper.Incubate(reaction, p.InactivationTemp, p.InactivationTime, false)
// all done
r.Reaction = reaction
}
func (e *Sum) validation(p SumParamBlock, r *SumResultBlock) {
_wrapper := execution.NewWrapper(p.ID)
_ = _wrapper
}
// Actions to perform after steps block to analyze data
func (e *Sum) analysis(p SumParamBlock, r *SumResultBlock) {
_wrapper := execution.NewWrapper(p.ID)
_ = _wrapper
}
// Core process of the protocol: steps to be performed for each input
func (e *Sum) steps(p SumParamBlock, r *SumResultBlock) {
_wrapper := execution.NewWrapper(p.ID)
_ = _wrapper
r.Sum = p.A + p.B
}
// Actions to perform before protocol itself
func (e *Sum) setup(p SumParamBlock) {
_wrapper := execution.NewWrapper(p.ID)
_ = _wrapper
}
// A block of tests to perform to validate that the sample was processed correctly
// Optionally, destructive tests can be performed to validate results on a
// dipstick basis
func (e *TypeIISConstructAssembly) validation(p TypeIISConstructAssemblyParamBlock, r *TypeIISConstructAssemblyResultBlock) {
_wrapper := execution.NewWrapper(p.ID)
_ = _wrapper
}
// Conditions to run on startup
func (e *TypeIISConstructAssembly) setup(p TypeIISConstructAssemblyParamBlock) {
_wrapper := execution.NewWrapper(p.ID)
_ = _wrapper
}