示例#1
0
文件: packed.go 项目: frogs/biogo
// Join p to the sequence at the end specified by where.
func (self *Seq) Join(p *Seq, where int) (err error) {
	if self.circular {
		return bio.NewError("Cannot join circular sequence: receiver.", 0, self)
	} else if p.circular {
		return bio.NewError("Cannot join circular sequence: parameter.", 0, p)
	}

	switch where {
	case seq.Start:
		p = p.Copy().(*Seq)
		p.S.Align(seq.End)
		self.S.Align(seq.Start)
		self.S.LeftPad = p.S.LeftPad
	case seq.End:
		p = p.Copy().(*Seq)
		p.S.Align(seq.Start)
		self.S.Align(seq.End)
		self.S.RightPad = p.S.RightPad
	default:
		return bio.NewError("Undefined location.", 0, where)
	}

	tt, offset := sequtils.Join(self.S.Letters, p.S.Letters, where)
	self.offset = offset
	self.S.Letters = tt.([]alphabet.Pack)

	return
}
示例#2
0
文件: fastq.go 项目: frogs/biogo
// Read a single sequence and return it or an error.
// TODO: Does not read multi-line fastq.
func (self *Reader) Read() (s seq.Sequence, err error) {
	var (
		buff, line, label []byte
		isPrefix          bool
		seqBuff           []alphabet.QLetter
		t                 seqio.SequenceAppender
	)

	inQual := false

	for {
		if buff, isPrefix, err = self.r.ReadLine(); err == nil {
			if isPrefix {
				line = append(line, buff...)
				continue
			} else {
				line = buff
			}

			line = bytes.TrimSpace(line)
			if len(line) == 0 {
				continue
			}
			switch {
			case !inQual && line[0] == '@':
				t = self.readHeader(line)
				label, line = line, nil
			case !inQual && line[0] == '+':
				if len(label) == 0 {
					return nil, bio.NewError("fastq: no header line parsed before +line in fastq format", 0)
				}
				if len(line) > 1 && bytes.Compare(label[1:], line[1:]) != 0 {
					return nil, bio.NewError("fastq: quality header does not match sequence header", 0)
				}
				inQual = true
			case !inQual:
				line = bytes.Join(bytes.Fields(line), nil)
				seqBuff = make([]alphabet.QLetter, len(line))
				for i := range line {
					seqBuff[i].L = alphabet.Letter(line[i])
				}
			case inQual:
				line = bytes.Join(bytes.Fields(line), nil)
				if len(line) != len(seqBuff) {
					return nil, bio.NewError("fastq: sequence/quality length mismatch", 0)
				}
				for i := range line {
					seqBuff[i].Q = alphabet.DecodeToQphred(line[i], self.enc)
				}
				t.AppendQLetters(seqBuff...)

				return t, nil
			}
		} else {
			return
		}
	}

	panic("cannot reach")
}
示例#3
0
文件: fastq.go 项目: frogs/biogo
// Write a single sequence and return the number of bytes written and any error.
func (self *Writer) Write(s *seq.Seq) (n int, err error) {
	if s.Quality == nil {
		return 0, bio.NewError("No quality associated with sequence", 0, s)
	}
	if s.Len() == s.Quality.Len() {
		self.template[1] = []byte(s.ID)
		self.template[3] = s.Seq
		if self.QID {
			self.template[4] = append(append([]byte("\n+"), []byte(s.ID)...), '\n')
		} else {
			self.template[4] = []byte("\n+\n")
		}
		self.template[5] = self.encodeQuality(s.Quality.Qual)
		var tn int
		for _, t := range self.template {
			tn, err = self.w.Write(t)
			n += tn
			if err != nil {
				return
			}
		}
	} else {
		return 0, bio.NewError("Sequence length and quality length do not match", 0, s)
	}

	return
}
示例#4
0
文件: promise.go 项目: frogs/biogo
func (p *Promise) fulfill(value interface{}) (err error) {
	r, set := p.messageState()

	if r.Err != nil {
		err = bio.NewError("Tried to fulfill a failed promise", 0, r.Err)
	} else {
		if !set || p.mutable {
			r.Value = value
			err = nil
		} else {
			err = bio.NewError("Tried to fulfill an already set immutable promise", 0)
		}
	}

	if err != nil && p.relay {
		if r.Err != nil {
			err = bio.NewError("Promise already failed - cannot relay", 0, r.Err)
		} else {
			r.Err = err
		}
	}

	p.message <- r

	return
}
示例#5
0
文件: seq.go 项目: frogs/biogo
func (self *Seq) Join(s *Seq, where int) (j *Seq, err error) {
	var (
		ts []byte
		ID string
	)

	if self.Circular {
		return nil, bio.NewError("Cannot join circular molecule.", 0, self)
	}

	if !self.Inplace && self.Quality != nil && self.Quality.Inplace {
		return nil, bio.NewError("Inplace operation on Quality with non-Inplace operation on parent Seq.", 0, self)
	}

	switch where {
	case Prepend:
		ID = s.ID + "+" + self.ID
		ts = make([]byte, len(s.Seq), len(s.Seq)+len(self.Seq))
		copy(ts, s.Seq)
		ts = append(ts, self.Seq...)
	case Append:
		ID = self.ID + "+" + s.ID
		if self.Inplace {
			ts = append(self.Seq, s.Seq...)
		} else {
			ts = make([]byte, len(self.Seq), len(s.Seq)+len(self.Seq))
			copy(ts, self.Seq)
			ts = append(ts, s.Seq...)
		}
	}

	var q *Quality
	if self.Quality != nil && s.Quality != nil {
		q, err = self.Quality.Join(s.Quality, where)
		if err != nil {
			return
		}
	}

	if self.Inplace {
		j = self
		j.ID = ID
		j.Seq = ts
		j.Quality = q // self.Quality will become nil if either sequence lacks Quality
	} else {
		j = &Seq{
			ID:      ID,
			Seq:     ts,
			Strand:  self.Strand,
			Moltype: self.Moltype,
			Quality: q,
		}
	}
	if where == Prepend {
		j.Offset -= s.Len()
	}

	return
}
示例#6
0
文件: seq.go 项目: frogs/biogo
func (self *Seq) Trunc(start, end int) (s *Seq, err error) {
	var ts []byte

	if !self.Inplace && self.Quality != nil && self.Quality.Inplace {
		return nil, bio.NewError("Inplace operation on Quality with non-Inplace operation on parent Seq.", 0, self)
	}

	if start < self.Offset || end < self.Offset ||
		start > len(self.Seq)+self.Offset || end > len(self.Seq)+self.Offset {
		return nil, bio.NewError("Start or end position out of range.", 0, self)
	}

	if start <= end {
		if self.Inplace {
			ts = self.Seq[start-self.Offset : end-self.Offset]
		} else {
			ts = append([]byte(nil), self.Seq[start-self.Offset:end-self.Offset]...)
		}
	} else if self.Circular {
		if self.Inplace {
			ts = append(self.Seq[start-self.Offset:], self.Seq[:end-self.Offset]...) // not quite inplace for this op
		} else {
			ts = make([]byte, len(self.Seq)-start-self.Offset, len(self.Seq)+end-start)
			copy(ts, self.Seq[start-self.Offset:])
			ts = append(ts, self.Seq[:end-self.Offset]...)
		}
	} else {
		return nil, bio.NewError("Start position greater than end position for non-circular molecule.", 0, self)
	}

	var q *Quality
	if self.Quality != nil {
		q, err = self.Quality.Trunc(start, end)
		if err != nil {
			err = bio.NewError("Quality.Trunc() returned error", 0, err)
			return
		}
	}

	if self.Inplace {
		s = self
		s.Seq = ts
		s.Circular = false
		s.Quality = q
	} else {
		s = &Seq{
			ID:       self.ID,
			Seq:      ts,
			Offset:   start,
			Strand:   self.Strand,
			Circular: false,
			Moltype:  self.Moltype,
			Quality:  q,
		}
	}

	return
}
示例#7
0
文件: letters.go 项目: frogs/biogo
// Pack a QLetter into a QPack. a.Len() == 4.
func (self QLetter) Pack(a Nucleic) (QPack, error) {
	if a.Len() != 4 {
		return 0, bio.NewError("Invalid alphabet", 0, self)
	}
	if !a.IsValid(self.L) {
		return QPack(byte(self.Q) << 2), bio.NewError("Invalid letter", 0, self)
	}
	return QPack(byte(self.Q)<<2 | byte(a.IndexOf(self.L)&0x3)), nil
}
示例#8
0
文件: pair.go 项目: frogs/biogo
// Convert coordinates in a packed sequence into a feat.Feature.
func featureOf(contigs *seq.Seq, from, to int, comp bool) (feature *feat.Feature, err error) {
	if comp {
		from, to = contigs.Len()-to, contigs.Len()-from
	}
	if from >= to {
		return nil, bio.NewError(fmt.Sprintf("%s: from > to", contigs.ID), 0, nil)
	}

	// DPHit coordinates sometimes over/underflow.
	// This is a lazy hack to work around it, should really figure
	// out what is going on.
	if from < 0 {
		from = 0
	}
	if to > contigs.Len() {
		to = contigs.Len()
	}

	// Take midpoint of segment -- lazy hack again, endpoints
	// sometimes under / overflow
	bin := (from + to) / (2 * binSize)
	binCount := (contigs.Len() + binSize - 1) / binSize

	if bin < 0 || bin >= binCount {
		return nil, bio.NewError(fmt.Sprintf("%s: bin %d out of range 0..%d", contigs.ID, bin, binCount-1), 0, nil)
	}

	contigIndex := contigs.Meta.(seqMap).binMap[bin]

	if contigIndex < 0 || contigIndex >= len(contigs.Meta.(seqMap).contigs) {
		return nil, bio.NewError(fmt.Sprintf("%s: contig index %d out of range 0..%d", contigs.ID, contigIndex, len(contigs.Meta.(seqMap).contigs)), 0, nil)
	}

	length := to - from

	if length < 0 {
		return nil, bio.NewError(fmt.Sprintf("%s: length < 0", contigs.ID), 0, nil)
	}

	contig := contigs.Meta.(seqMap).contigs[contigIndex]
	contigFrom := from - contig.from
	contigTo := contigFrom + length

	if contigFrom < 0 {
		contigFrom = 0
	}

	if contigTo > contig.seq.Len() {
		contigTo = contig.seq.Len()
	}

	return &feat.Feature{
		ID:    contig.seq.ID,
		Start: contigFrom,
		End:   contigTo,
	}, nil
}
示例#9
0
文件: seq.go 项目: frogs/biogo
// Join p to the sequence at the end specified by where.
func (self *Seq) Join(p *Seq, where int) (err error) {
	if self.circular {
		return bio.NewError("Cannot join circular sequence: receiver.", 1, self)
	} else if p.circular {
		return bio.NewError("Cannot join circular sequence: parameter.", 1, p)
	}

	tt, offset := sequtils.Join(self.S, p.S, where)
	self.offset = offset
	self.S = tt.([]alphabet.Letter)

	return
}
示例#10
0
文件: fastq.go 项目: frogs/biogo
// Read a single sequence and return it or an error.
// TODO: Does not read interleaved fastq.
func (self *Reader) Read() (sequence *seq.Seq, err error) {
	var line, label, seqBody, qualBody []byte
	sequence = &seq.Seq{}

	inQual := false
READ:
	for {
		line, err = self.r.ReadBytes('\n')
		if err == nil {
			if len(line) > 0 && line[len(line)-1] == '\r' {
				line = line[:len(line)-1]
			}
			line = bytes.TrimSpace(line)
			if len(line) == 0 {
				continue
			}
			switch {
			case !inQual && line[0] == '@':
				label = line[1:]
			case !inQual && line[0] == '+':
				if len(label) == 0 {
					return nil, bio.NewError("No ID line parsed at +line in fastq format", 0)
				}
				if len(line) > 1 && bytes.Compare(label, line[1:]) != 0 {
					return nil, bio.NewError("Quality ID does not match sequence ID", 0)
				}
				inQual = true
			case !inQual:
				line = bytes.Join(bytes.Fields(line), nil)
				seqBody = append(seqBody, line...)
			case inQual:
				line = bytes.Join(bytes.Fields(line), nil)
				qualBody = append(qualBody, line...)
				if len(qualBody) >= len(seqBody) {
					break READ
				}
			}
		} else {
			return
		}
	}

	if len(seqBody) != len(qualBody) {
		return nil, bio.NewError("Quality length does not match sequence length", 0)
	}

	labelString := string(label)
	sequence = seq.New(labelString, seqBody, seq.NewQuality(labelString, self.decodeQuality(qualBody)))

	return
}
示例#11
0
文件: qpacked.go 项目: frogs/biogo
// Join p to the sequence at the end specified by where.
func (self *QSeq) Join(p *QSeq, where int) (err error) {
	if self.circular {
		return bio.NewError("Cannot join circular sequence: receiver.", 1, self)
	} else if p.circular {
		return bio.NewError("Cannot join circular sequence: parameter.", 1, p)
	}

	var tt interface{}

	tt, self.offset = sequtils.Join(self.S, p.S, where)
	self.S = tt.([]alphabet.QPack)

	return
}
示例#12
0
文件: kmerindex.go 项目: frogs/biogo
// Convert a string of bases into a Kmer, returns an error if string length does not match word length
func (self *Index) KmerOf(kmertext string) (kmer Kmer, err error) {
	if len(kmertext) != self.k {
		return 0, bio.NewError("Sequence length does not match Kmer length", 0, self.k, kmertext)
	}

	for _, v := range kmertext {
		x := lookUp.ValueToCode[v]
		if x < 0 {
			return 0, bio.NewError("Kmer contains illegal character", 0, kmertext)
		}
		kmer = (kmer << 2) | Kmer(x)
	}

	return
}
示例#13
0
文件: packed.go 项目: frogs/biogo
func checkPackedAlpha(alpha alphabet.Nucleic) error {
	if alpha.Len() != 4 {
		return bio.NewError("Cannot create packed sequence with alphabet length != 4", 0, alpha)
	}
	for _, v := range alphabet.BytesToLetters([]byte(alpha.String())) {
		if c, ok := alpha.Complement(v); ok && alpha.IndexOf(v) != alpha.IndexOf(c)^0x3 {
			// TODO: Resolution to the following problem:
			// Normal nucleotide alphabets (ACGT/ACGU) are safe with this in either case sensitive or
			// insensitive. Other alphabets may not be, in this case specify case sensitive.
			return bio.NewError("alphabet order not consistent with bit operations for packed.", 0, alpha)
		}
	}

	return nil
}
示例#14
0
文件: kmerindex.go 项目: frogs/biogo
// Return an array of positions for the Kmer string kmertext
func (self *Index) GetPositionsString(kmertext string) (positions []int, err error) {
	switch {
	case len(kmertext) != self.k:
		return nil, bio.NewError("Sequence length does not match Kmer length", 0, self.k, kmertext)
	case !self.indexed:
		return nil, bio.NewError("Index not built: call Build()", 0, self)
	}

	var kmer Kmer
	if kmer, err = self.KmerOf(kmertext); err != nil {
		return nil, err
	}

	return self.GetPositionsKmer(kmer)
}
示例#15
0
文件: alignment.go 项目: frogs/biogo
func NewSeq(id string, subids []string, b [][]alphabet.Letter, alpha alphabet.Peptide, cons protein.Consensifyer) (*Seq, error) {
	switch lids, lseq := len(subids), len(b); {
	case lids == 0 && len(b) == 0:
	case lseq != 0 && lids == len(b[0]):
		if lids == 0 {
			subids = make([]string, len(b[0]))
			for i := range subids {
				subids[i] = fmt.Sprintf("%s:%d", id, i)
			}
		}
	default:
		return nil, bio.NewError("alignment: id/seq number mismatch", 0)
	}

	return &Seq{
		ID:         id,
		SubIDs:     append([]string(nil), subids...),
		S:          append([][]alphabet.Letter(nil), b...),
		alphabet:   alpha,
		Consensify: cons,
		Stringify: func(s seq.Polymer) string {
			t := s.(*Seq).Consensus(false)
			return t.String()
		},
	}, nil
}
示例#16
0
文件: gff.go 项目: frogs/biogo
func (self *Reader) metaSequence(moltype, id string) (sequence *seq.Seq, err error) {
	var line, body []byte

	for {
		line, err = self.r.ReadBytes('\n')
		if err != nil {
			return nil, err
		}
		if len(line) > 0 && line[len(line)-1] == '\r' {
			line = line[:len(line)-1]
		}
		if len(line) == 0 {
			continue
		}
		if len(line) < 2 || !bytes.HasPrefix(line, []byte("##")) {
			return nil, bio.NewError("Corrupt metasequence", 0, line)
		}
		line = bytes.TrimSpace(line[2:])
		if string(line) == "end-"+moltype {
			break
		} else {
			line = bytes.Join(bytes.Fields(line), nil)
			body = append(body, line...)
		}
	}

	sequence = seq.New(id, body, nil)
	sequence.Moltype = bio.ParseMoltype(moltype)

	return
}
示例#17
0
文件: qalignment.go 项目: frogs/biogo
func NewQSeq(id string, subids []string, ql [][]alphabet.QLetter, alpha alphabet.Nucleic, encode alphabet.Encoding, cons nucleic.Consensifyer) (*QSeq, error) {
	switch lids, lseq := len(subids), len(ql); {
	case lids == 0 && len(ql) == 0:
	case lseq != 0 && lids == len(ql[0]):
		if lids == 0 {
			subids = make([]string, len(ql[0]))
			for i := range subids {
				subids[i] = fmt.Sprintf("%s:%d", id, i)
			}
		}
	default:
		return nil, bio.NewError("alignment: id/seq number mismatch", 0)
	}

	return &QSeq{
		ID:         id,
		SubIDs:     append([]string(nil), subids...),
		S:          append([][]alphabet.QLetter(nil), ql...),
		alphabet:   alpha,
		encoding:   encode,
		Strand:     1,
		Consensify: cons,
		Threshold:  2,
		LowQFilter: func(s seq.Sequence, _ alphabet.Letter) alphabet.Letter { return s.Alphabet().Ambiguous() },
		Stringify: func(s seq.Polymer) string {
			t := s.(*QSeq).Consensus(false)
			t.Threshold = s.(*QSeq).Threshold
			t.LowQFilter = s.(*QSeq).LowQFilter
			return t.String()
		},
	}, nil
}
示例#18
0
文件: packed.go 项目: frogs/biogo
// Join segments of the sequence, returning any error.
func (self *Seq) Compose(f feat.FeatureSet) (err error) {
	l := 0
	for _, seg := range f {
		if seg.End < seg.Start {
			return bio.NewError("Feature end < start", 0, seg)
		}
		l += util.Min(seg.End, self.End()) - util.Max(seg.Start, self.Start())
	}

	t := &Seq{}
	*t = *self
	t.S = &Packing{Letters: make([]alphabet.Pack, 0, (l+3)/4)}

	var tseg seq.Sequence
	for _, seg := range f {
		tseg, err = self.Subseq(util.Max(seg.Start, self.Start()), util.Min(seg.End, self.End()))
		if err != nil {
			return
		}
		tseg := tseg.(*Seq)
		if seg.Strand == -1 {
			tseg.RevComp()
		}
		tseg.S.Align(seq.Start)
		t.S.Align(seq.End)
		t.S.Letters = append(t.S.Letters, tseg.S.Letters...)
		t.S.RightPad = tseg.S.RightPad
	}

	*self = *t

	return
}
示例#19
0
文件: map.go 项目: frogs/biogo
// Map routines to iterate a function over an array, potentially splitting the array slice into
// chunks so that each chunk is processed concurrently. When using concurrent processing the
// Chunk size is either the nearest even division of the total array over the chosen concurrent
// processing goroutines or a specified maximum chunk size, whichever is smaller. Reducing
// chunk size can reduce the impact of divergence in time for processing chunks, but may add
// to overhead.
func Map(set Mapper, threads, maxChunkSize int) (results []interface{}, err error) {
	queue := make(chan Operator, 1)
	p := NewProcessor(queue, 0, threads)
	defer p.Stop()

	chunkSize := util.Min(int(math.Ceil(float64(set.Len())/float64(threads))), maxChunkSize)

	quit := make(chan struct{})

	go func() {
		for s := 0; s*chunkSize < set.Len(); s++ {
			select {
			case <-quit:
				break
			default:
				endChunk := util.Min(chunkSize*(s+1), set.Len())
				queue <- set.Slice(chunkSize*s, endChunk)
			}
		}
	}()

	for r := 0; r*chunkSize < set.Len(); r++ {
		result := <-p.out
		if result.Err != nil {
			err = bio.NewError("Map failed", 0, err)
			close(quit)
			break
		}
		results = append(results, result.Value)
	}

	return
}
示例#20
0
文件: multi.go 项目: frogs/biogo
func (self *Multi) Join(a *Multi, where int) (err error) {
	if self.Count() != a.Count() {
		return bio.NewError("Multis do not hold the same number of sequences", 0, []*Multi{self, a})
	}

	switch where {
	case seq.Start:
		if !a.IsFlush(seq.End) {
			a.Flush(seq.End, self.alphabet.Gap())
		}
		if !self.IsFlush(seq.Start) {
			self.Flush(seq.Start, self.alphabet.Gap())
		}
	case seq.End:
		if !a.IsFlush(seq.Start) {
			a.Flush(seq.Start, self.alphabet.Gap())
		}
		if !self.IsFlush(seq.End) {
			self.Flush(seq.End, self.alphabet.Gap())
		}
	}

	for i := 0; i < self.Count(); i++ {
		s := self.Get(i)
		as := a.Get(i)
		err = joinOne(s, as, where)
		if err != nil {
			return
		}
	}

	return
}
示例#21
0
文件: multi.go 项目: frogs/biogo
func joinOne(s, as protein.Sequence, where int) (err error) {
	switch s.(type) {
	case *protein.Seq:
		if t, ok := as.(*protein.Seq); !ok {
			err = joinFailure(s, t)
		} else {
			err = s.(*protein.Seq).Join(t, where)
		}
	case *protein.QSeq:
		if t, ok := as.(*protein.QSeq); !ok {
			err = joinFailure(s, t)
		} else {
			err = s.(*protein.QSeq).Join(t, where)
		}
	case *Multi:
		if t, ok := as.(*Multi); !ok {
			err = joinFailure(s, t)
		} else {
			err = s.(*Multi).Join(t, where)
		}
	default:
		joinerRegistryLock.RLock()
		if joinerFunc, ok := joinerRegistry[reflect.TypeOf(s)]; ok {
			err = joinerFunc(s, as, where)
		} else {
			err = bio.NewError(fmt.Sprintf("Sequence type %T not handled.", s), 0, s)
		}
		joinerRegistryLock.RUnlock()
	}

	return
}
示例#22
0
文件: gff.go 项目: frogs/biogo
// Rewind the reader.
func (self *Reader) Rewind() (err error) {
	if s, ok := self.f.(io.Seeker); ok {
		_, err = s.Seek(0, 0)
	} else {
		err = bio.NewError("Not a Seeker", 0, self)
	}
	return
}
示例#23
0
文件: utils.go 项目: frogs/biogo
// Truncate provides a function that may be used by polymer types to implement Truncator.
// It makes use of reflection and so may be slower than type-specific implementations.
// This is the reference implementation and should be used to compare type-specific
// implementation against in testing.
func Truncate(pol interface{}, start, end int, circular bool) (p interface{}, err error) {
	pv := reflect.ValueOf(pol)
	if l := pv.Len(); start < 0 || end < 0 || start > l || end > l {
		return nil, bio.NewError("Out of range.", 0, nil)
	}
	if start <= end {
		p = pv.Slice(start, end).Interface()
	} else if circular {
		tv := reflect.MakeSlice(pv.Type(), pv.Len()-start, pv.Len()+end-start)
		reflect.Copy(tv, pv.Slice(start, pv.Len()))
		p = reflect.AppendSlice(tv, pv.Slice(0, end)).Interface()
	} else {
		return nil, bio.NewError("Start position greater than end position for non-circular sequence.", 0, pol)
	}

	return
}
示例#24
0
文件: seq.go 项目: frogs/biogo
func (self *Seq) RevComp() (s *Seq, err error) {
	var rs []byte
	if self.Inplace {
		rs = self.Seq
	} else {
		if self.Quality != nil && self.Quality.Inplace {
			return nil, bio.NewError("Inplace operation on Quality with non-Inplace operation on parent Seq.", 0, self)
		}
		rs = make([]byte, len(self.Seq))
	}

	if self.Moltype == bio.DNA || self.Moltype == bio.RNA {
		i, j := 0, len(self.Seq)-1
		for ; i < j; i, j = i+1, j-1 {
			rs[i], rs[j] = complement[self.Moltype][self.Seq[j]], complement[self.Moltype][self.Seq[i]]
		}
		if i == j {
			rs[i] = complement[self.Moltype][self.Seq[i]]
		}
	} else {
		return nil, bio.NewError("Cannot reverse-complement protein.", 0, self)
	}

	var q *Quality
	if self.Quality != nil {
		q = self.Quality.Reverse()
	}

	if self.Inplace {
		s = self
		s.Quality = q
	} else {
		s = &Seq{
			ID:       self.ID,
			Seq:      rs,
			Offset:   self.Offset + len(self.Seq),
			Strand:   -self.Strand,
			Circular: self.Circular,
			Moltype:  self.Moltype,
			Quality:  q,
		}
	}

	return
}
示例#25
0
文件: fasta.go 项目: frogs/biogo
// Rewind the reader.
func (self *Reader) Rewind() (err error) {
	if s, ok := self.f.(io.Seeker); ok {
		self.last = nil
		_, err = s.Seek(0, 0)
		self.r = bufio.NewReader(self.f)
	} else {
		err = bio.NewError("Not a Seeker", 0, self)
	}
	return
}
示例#26
0
文件: align.go 项目: frogs/biogo
// Returns the sums of alignment lengths.
func (h DPHits) Sum() (a, b int, err error) {
	for _, hit := range h {
		la, lb := hit.Aepos-hit.Abpos, hit.Bepos-hit.Bbpos
		if la < 0 || lb < 0 {
			return 0, 0, bio.NewError("Area < 0", 0, hit)
		}
		a, b = a+la, b+lb
	}
	return
}
示例#27
0
文件: multi.go 项目: frogs/biogo
func (self *Multi) Add(n ...protein.Sequence) (err error) {
	for _, s := range n {
		if s.Alphabet() != self.alphabet {
			return bio.NewError("Inconsistent alphabets", 0, self, s)
		}
	}
	self.S = append(self.S, n...)

	return
}
示例#28
0
文件: packed.go 项目: frogs/biogo
// Append QLetters to the sequence.
func (self *Seq) AppendQLetters(a ...alphabet.QLetter) (err error) {
	defer func() {
		if r := recover(); r != nil {
			_, pos := self.alphabet.AllValidQLetter(a)
			err = bio.NewError(fmt.Sprintf("Encoding error: %s %q at position %d.", r, a[pos], pos), 1, a)
		}
	}()

	i := 0
	for ; self.S.RightPad > 0 && i < len(a); i, self.S.RightPad = i+1, self.S.RightPad-1 {
		if !self.alphabet.IsValid(a[i].L) {
			return bio.NewError(fmt.Sprintf("Invalid letter %q at position %d.", a[i], i), 0, nil)
		}
		self.S.Letters[len(self.S.Letters)-1] |= alphabet.Pack(self.alphabet.IndexOf(a[i].L)) << (4 - byte(self.S.RightPad))
	}
	self.S.Letters = append(self.S.Letters, PackQLetters(self.alphabet, a[i:]...).Letters...)

	return
}
示例#29
0
文件: qual.go 项目: frogs/biogo
func (self *Quality) Trunc(start, end int) (q *Quality, err error) {
	var tq []Qsanger

	if start < self.Offset || end < self.Offset ||
		start > len(self.Qual)+self.Offset || end > len(self.Qual)+self.Offset {
		return nil, bio.NewError("Start or end position out of range.", 0, self)
	}

	if start <= end {
		if self.Inplace {
			tq = self.Qual[start-self.Offset : end-self.Offset]
		} else {
			tq = append([]Qsanger(nil), self.Qual[start-self.Offset:end-self.Offset]...)
		}
	} else if self.Circular {
		if self.Inplace {
			tq = append(self.Qual[start-self.Offset:], self.Qual[:end-self.Offset]...) // not quite inplace for this op
		} else {
			tq = make([]Qsanger, len(self.Qual)-start-self.Offset, len(self.Qual)+end-start)
			copy(tq, self.Qual[start-self.Offset:])
			tq = append(tq, self.Qual[:end-self.Offset]...)
		}
	} else {
		return nil, bio.NewError("Start position greater than end position for non-circular molecule.", 0, self)
	}

	if self.Inplace {
		q = self
		q.Qual = tq
		q.Circular = false
	} else {
		q = &Quality{
			ID:       self.ID,
			Qual:     tq,
			Offset:   start,
			Strand:   self.Strand,
			Circular: false,
		}
	}

	return
}
示例#30
0
文件: qalignment.go 项目: frogs/biogo
// Append each byte of each a to the appropriate sequence in the reciever.
func (self *QSeq) AppendColumns(a ...[]alphabet.QLetter) (err error) {
	for i, s := range a {
		if len(s) != self.Count() {
			return bio.NewError(fmt.Sprintf("Column %d does not match Count(): %d != %d.", i, len(s), self.Count()), 0, a)
		}
	}

	self.S = append(self.S, a...)

	return
}